Title: Production of resistant lines to Glyphosate, stem borer, and blast through physical and chemical mutation induction in rice
Author: Aliakbar Ebadi
Abstract: In this project, it was used from the landrace varieties Deylamani, Hashmi, Binam, and Ali Kazemi as plant materials. In order to determine the optimum dose, seeds were irradiated with different doses (0, 200, 250, 300, 350, 400 Gy) in Gamma Cell. LD50 for the survival of seedlings was calculated in different doses for all genotypes. Then, M1 generation plant populations of different genotypes were established after irradiation with selected optimum doses. It was harvested a spike from any M1 plant. In the population of M2, plants were selected based on morphological characteristics. In the third year, 344 plants were selected based on morphological characteristics of the M3 population. All selected plants were cultivated as selective M4 lines in two separate plots. In one of them, all cultivated plants were treated with Glyphosate herbicide, but unfortunately, no tolerant plant was detected. In the next plot, all lines were evaluated for resistance to blast disease, and 75 lines were selected. Selected lines were cultivated as M5 generations with 4 parents as control cultivar with 5-replicate based on augmented design. The results showed that the effect of treatment for all studied traits was significant at a 1% level, which indicated good variation due to mutation. Also, the level of tolerance to the stem borer in most of the mutant lines was superior to that of parental cultivars. Based on the yield trait and other quantitative and qualitative traits, 14 mutant lines (9 mutant lines from Tarom Deylmani, 3 mutant lines from Alikazmi, 2 mutant lines from Hashemi, and 2 mutant lines from Binam) were selected. Selected mutant lines will be evaluated next year.
Keywords: Rice, Mutation, Resistance to blast disease