Title: Evaluation of antioxidant activities and seed yield of internal and external lines and cultivars of rice (Oryza sativa L.) cultivated in Mazandaran province
Author: Mohammad Taghi Karbalaei Aghamolki
Abstract: In order to study the quantitative and anti-oxidant properties of ten lines and colored seeds with Hashemi local and high yielding h three replications at Goldasht Research Station (Rice Research InstituFajr, An experiment was conducted in a randomized complete block design in 2017. The results showed that the Fajr cultivar had a number of fertile tillers per plant, more grain number per panicle, and filled grain than other genotypes, but had a lower 1000-gram weight. Also, Fajr and KR13 cultivars had the highest grain yield with 6583.3 and 4716.7 kg ha-1, respectively. White rice, brown rice, husk, and bran of Fajr, Hashemi, and KB13 three rice varieties were scavenging and reducing power. The total phenolic and flavonoid contents were also determined. Ferulic acid and p-Coumaric acid were detected by High-performance liquid chromatography (HPLC). KB13 rice bran showed the highest extracted by maceration method using methanol as a solvent. Antioxidant capacity was assessed by utilizing three different methods: DPPH and nitric oxide (NO) free radicals amount of total phenolics (319.83 ± 15.21 GAE) and flavonoids contents (77.58 ± 3.80 QE). In DPPH radical scavenging activity, the Hashemi crude bran extract had the same activity as Fajr and KB13 crude brans but this activity was significantly different from other extracts. In reducing power assay, at 100-800 g/ml, Fajr white rice showed the highest activity which was significantly higher than other extracts. In nitric oxide radical scavenging activity, the barn of three rice varieties showed the highest activity. Hashemi bran with 26.21 and KB13 bran with 3.66 mg/g extract showed the highest amount of Ferulic acid and p-Coumaric acid, respectively. The results of this study showed that the Fajr cultivar had more grain yield than other genotypes. Also, the highest antioxidant activity was in terms of total phenolic and flavonoid content belonged to KB13, and Fajr and Hashemi cultivars were in the next category cultivars.
Keywords: Antioxidant, Flavonoid, Genotype, Rice, Yield